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文献和实验Isolation of Covalent Enzyme-DNA Complexes
present in the active site of the enzyme. A novel aspect of the breakagereunion reaction is the the formation of a transient covalent O 4 -phosphotyrosyl bond between the topoisomerase protein and a phosphate residue present at the break site in the DNA
Isolation of a Membrane-Bound Enzyme, 5-Nucleotidase
(2 ). The purification of a membrane enzyme thus requires two additional considerations compared to the isolation of a cytosolic enzyme: the isolation of a membrane fraction containing the enzyme and the solubilization of the enzyme from this fraction
CASK: A Specialized Neuronal Kinase
CASK, a scaffolding protein present in neuronal synapses and other cell junctions, contains a CaM-kinase domain at its N terminus. Due to lack of adequate biochemical data and based on bioinformatics, this domain was classified
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