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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Pure form: -20°C, 3 years.In solvent: -80°C, 6 months; -20°C, 1 month.
- 英文名:
13-Azido-2,5,8,11-tetraoxatridecane
- 库存:
货期:1-2天
- 供应商:
MedChemExpress LLC
- CAS号:
606130-90-9
- 规格:
50 mg/100 mg
| 规格: | 50 mg | 产品价格: | ¥420.0 |
|---|---|---|---|
| 规格: | 100 mg | 产品价格: | ¥700.0 |
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m-PEG4-azide
CAS No. : 606130-90-9
MCE 国际站:m-PEG4-azide
产品活性:m-PEG4-azide (13-Azido-2,5,8,11-tetraoxatridecane) 是一种 PROTAC linker,属于 PEG 类,可用于合成 PROTAC。m-PEG4-azide 是一种点击化学试剂。它含有 Azide 基团,可以和含有 Alkyne 基团的分子发生铜催化的叠氮-炔环加成反应(CuAAc)。还可以和含有 DBCO 或 BCN 基团的分子发生菌株促进的炔-叠氮环加成反应 (SPAAC)。
研究领域:PROTAC
作用靶点:PROTAC Linkers
In Vitro: PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
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文献和实验Replication timing by density transfer
for ~1 min. You can empirically determine how long it takes to bring your desired volume of medium from 37o to 23o C. 6. Collecting samples: Freeze 8 ml of 0.1% Na-azide, 0.2 M EDTA in "50 ml" Oakridge-type plastic, screw-capped centrifuge tubes
Replication timing by comparative hybridization
Replication timing by comparative hybridization M. K. Raghuraman Reference: Friedman, K. L., M. K. Raghuraman, W. L. Fangman, and B. J. Brewer (1995) Analysis of the temporal program of replication initiation
Purification of Antiserum or Ascites by Protein A/G Chromatography
1、Required Materials and Equipment(1) Protein A or G agarose gel column (10 ml or 5 ml of packed beads; see guidelines below for choice of protein A or protein G)(2) Ice-cold Tris-buffered saline (TBS). See recipe below(3) 5% sodium azide solution
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