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Azide-PEG16-alcohol
MCE 国际站：Azide-PEG16-alcohol
产品活性：Azide-PEG16-alcohol 是一种 PROTAC linker，属于 PEG 类。可用于合成 PROTAC 分子。Azide-PEG16-alcohol 是一种点击化学试剂。它含有 Azide 基团，可以和含有 Alkyne 基团的分子发生铜催化的叠氮-炔环加成反应（CuAAc)。还可以和含有 DBCO 或 BCN 基团的分子发生菌株促进的炔-叠氮环加成反应 (SPAAC)。
研究领域：PROTAC
作用靶点：PROTAC Linkers
In Vitro: PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
相关产品：5-Ethynyl-2'-deoxyuridine | TGN-020 | NH2-C2-NH-Boc | DBCO-PEG4-NHS ester | L-Azidohomoalanine hydrochloride | N-Boc-piperazine | 3-Mercaptopropionic acid NHS ester | Alkynyl Palmitic Acid | Boc-NH-PEG3 | Amino-PEG3-C2-Azido | Biotin-PEG3-azide | Fmoc-8-amino-3,6-dioxaoctanoic acid | NH2-PEG2-C6-Cl | 3,6-Dioxaoctanedioic acid | EGNHS | Leukotriene B4 | NH2-PEG3 | Biotin alkyne | Boc-NH-PEG2-CH2COOH | Fmoc-NH-PEG4-CH2CH2COOH | Ac4GalNAz | Azido-PEG3-alcohol | Biotin-PEG4-NHS ester | Biotin-PEG4-alkyne | Boc-NH-C4-Br | DBCO-PEG5-NHS ester | L-Homopropargylglycine hydrochloride | NH2-PEG3-C2-NH-Boc | 2-((Azido-PEG8-carbamoyl)methoxy)acetic acid | 3-Maleimidopropionic acid
热门产品线：重组蛋白 | 化合物库 | 天然产物 | 荧光染料 | PROTAC | 同位素标记物
Trending products：Recombinant Proteins | Bioactive Screening Libraries | Natural Products | Dye Reagents | PROTAC | Isotope-Labeled Compounds
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文献和实验of the supernatant and add 0.7 mL of 70% ethyl alcohol.14. Spin the tube at 14,000 rpm for 10 minutes.15. Discard the supernatant and dry the pellet in the vacuum for 10 minutes.16. Dissolve the pellet in 50 mL of TE buffer.17. Load 10 mL of your sample
Replication timing by density transfer
, leu2-3,112, ura3-52, his6 in an A364A background. I aim for about 32 x 106 cells per time point. That gives enough DNA for at least 3 blots, leaving enough leeway for errors. 2. When the cell density is 2 x 106 cells/ml (OD660 ~ 0.16
the pellet by repeated pipetting. This may take some work. Transfer the suspended phage to a microfuge tube. 11. Extract twice with 1 volume of chloroform (containing no isoamyl alcohol!). This step removes some of the PEG while the phage are still intact
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