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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Powder: -20°C, 3 years; 4°C, 2 years.In solvent: -80°C, 6 months; -20°C, 1 month.
- 库存：
货期：1-2天
- 供应商：
MedChemExpress LLC
- 规格：
10 mM * 1 mL/1 mg/5 mg/10 mg/25 mg/50 mg
| 规格： | 10 mM * 1 mL | 产品价格： | ￥1513.0 |
|---|---|---|---|
| 规格： | 1 mg | 产品价格： | ￥550.0 |
| 规格： | 5 mg | 产品价格： | ￥1375.0 |
| 规格： | 10 mg | 产品价格： | ￥2200.0 |
| 规格： | 25 mg | 产品价格： | ￥3300.0 |
| 规格： | 50 mg | 产品价格： | ￥4620.0 |
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MLS000544460
CAS No. : 352336-36-8
MCE 国际站：MLS000544460
产品活性：MLS000544460 是一种高度选择性和可逆的 Eya2 磷酸酶抑制剂，Kd 为 2.0 μM，IC50 为 4 μM。MLS000544460 抑制 Eya2 磷酸酶介导的细胞迁移，并具有抗癌活性。
研究领域：Metabolic Enzyme/Protease
作用靶点：Phosphatase
In Vitro: MLS000544460 does not bind Eya3 phosphatase (ED).
MLS000544460 (10 μM) inhibits Eya2-mediated cell migration in human Eya2, Eya2 phosphatase-dead (D274N), and YFP (as a negative control) expressing MCF10A cells.
When Eya2 ED is dialyzed into 10 μM EDTA to remove Mg2+, it binds MLS000544460 with a higher affinity (Kd=0.80 μM), indicating that this class of compounds does not require Mg2+ for its interaction with the Eya2 ED.
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文献和实验Preparing 48-Well Plate Feeders
/plate). Example: For a total of 5 x 48-well plates, the calculation is: 5 plates x 31.2 mls = 156 mls + 10 mls Extra = 166 mls (Total) 166 mls x 8.2 x 104 cells/ml = 1.36 x 107 cells (Total) 1.36 x 107 cells : 3.5 x 105 cells
Preparing 48-Well Plate Feeders[Baylor College of Medicine]
, the calculation is: 5 plates x 31.2 mls = 156 mls + 10 mls Extra = 166 mls (Total) 166 mls x 8.2 x 104 cells/ml = 1.36 x 107 cells (Total) 1.36 x 107 cells : 3.5 x 105 cells/ml = 38.86 mls Dilution: 38.9 mls (of cell suspension @ 3.5 x
SILVER STAIN OF PROTEIN GELS(Ⅱ)
of 0.36% NaOH plus1.4 mls of concentrated NH4OH (a little extra may be needed; add just enough to get silver to dissolve wait for lag!)then bring up to 100 mls with dH2O (by adding 72.8 mls) Developer 500 mls of dH2O 2.5 mls of 1% Citric Acid
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