手机验证
发货周期:现货
博士德生物
cell culture supernates, serum and plasma (heparin, EDTA).
Rat
96T
货号 | EK2086 |
---|---|
产品名称 | Rat Chordin ELISA Kit |
产品描述 | For quantitative detection of rat Chordin in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, T27-S948 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | Chrd |
基因全名 | chordin |
Uniprot ID | Q63148 |
指标别名 | Chordin; Chrd |
背景资料 | Chordin is a bone morphogenetic protein antagonist composed of four small cysteine-rich domains, whose function is not known. In humans, the chordin peptide is encoded by the CHRD gene. It is mapped to 11q23. It dorsalizes the developing embryo by binding ventralizing TGFβ proteins such as bone morphogenetic proteins. It may also play a role in organogenesis. In mice, Chordin is expressed in the node but not in the anterior visceral endoderm. It has been found to be required for forebrain development. In developing mice that are deficient in both chordin and noggin, the head is nearly absent. This is significant because when only noggin is deficient there are mild defects but the head still forms. Chordin is also involved in avian gastrulation. It is expressed in the anterior cells of Koller's sickle, which form the anterior cells of the primitive streak, a key structure through which gastrulation occurs. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2086-CAP | 96-well plate precoated with anti- rat Chordin antibody | 1 |
EK2086-ST | Lyophilized recombinant rat Chordin standard | 10ng/tube×2 |
EK2086-DA | Biotinylated anti- rat Chordin antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1. 96孔读板仪器。
2. 自动洗板机.
3. 移液器和枪头。建议使用多通道移液器/排枪。
4. Eppendorf 管。
5. 额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 95 | 293 | 1596 | 100 | 395 | 1791 |
Standard deviation | 4.85 | 15.53 | 89.38 | 6.60 | 26.86 | 127.16 |
CV(%) | 5.1% | 5.3% | 5.6% | 6.6% | 6.8% | 7.1% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.152 | 0.173 | 0.225 | 0.311 | 0.496 | 0.819 | 1.297 | 2.064 |
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