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发货周期:现货
博士德生物
cell culture supernates, serum and plasma (heparin, EDTA).
Mouse
96T
货号 | EK1645 |
---|---|
产品名称 | Mouse HAVCR2 ELISA Kit |
产品描述 | For Quantitative Detection of mouse TIM-3 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO,R20-A193 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | HAVCR2 |
基因全名 | hepatitis A virus cellular receptor 2 |
Uniprot ID | HAVCR2: Q8VIM0 |
指标别名 | Hepatitis A virus cellular receptor 2 homolog; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3; Havcr2; Tim3; Timd3 |
背景资料 | Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in mouses is encoded by the HAVCR2 gene. The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1645-CAP | 96-well plate precoated with anti- mouse TIM-3 antibody | 1 |
EK1645-ST | Lyophilized recombinant mouse TIM-3 standard | 10ng/tube×2 |
EK1645-DA | Biotinylated anti- mouse TIM-3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 78 | 223 | 1009 | 80 | 239 | 1060 |
Standard deviation | 5.69 | 13.38 | 41.36 | 6.88 | 17.2 | 53 |
CV(%) | 7.3% | 6% | 4.1% | 8.6% | 7.2% | 5% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.005 | 0.071 | 0.150 | 0.301 | 0.575 | 1.078 | 1.679 | 2.230 |
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