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发货周期:现货
博士德生物
cell culture supernates, serum and plasma (heparin, EDTA).
Mouse
96T
货号 | EK1420 |
---|---|
产品名称 | Mouse CFD ELISA Kit |
产品描述 | For Quantitative Detection of mouse CFD in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli,I26-S259 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <20pg/ml |
检测范围 | 0.78ng/ml-50ng/ml |
基因名称 | Cfd |
基因全名 | complement factor D (adipsin) |
Uniprot ID | P03953 |
指标别名 | Complement factor D; 28 kDa adipocyte protein; Adipsin; C3 convertase activator; Properdin factor D; Cfd; And; Df |
背景资料 | Complement factor D (CFD), also called ADIPSIN or FACTOR D, is a protein which in humans is encoded by the CFD gene. The protein encoded by this gene belongs to the trypsin family of peptidases. It is mapped to 19p13.3. The encoded protein is a component of the alternative complement pathway which is best known for its role in humoral suppression of infectious agents. This protein is also a serine protease that is secreted by adipocytes into the bloodstream. Finally, the encoded protein has a high level of expression in fat, suggesting a role for adipose tissue in immune system biology. What’s more, Factor D is a serine protease that stimulates glucose transport for triglyceride accumulation in fats cells and inhibits lipolysis. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1420-CAP | 96-well plate precoated with anti- mouse CFD antibody | 1 |
EK1420-ST | Lyophilized recombinant mouse CFD standard | 50ng/tube×2 |
EK1420-DA | Biotinylated anti- mouse CFD antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1435 | 4423 | 19418 | 1328 | 4783 | 18803 |
Standard deviation | 107.62 | 309.61 | 1378.67 | 111.55 | 411.33 | 1541.84 |
CV(%) | 7.5% | 7% | 7.1% | 8.4% | 8.6% | 8.2% |
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.011 | 0.047 | 0.091 | 0.181 | 0.367 | 0.672 | 1.100 | 1.853 |
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