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发货周期:7-8周
博士德生物
cell culture supernates, serum and plasma (heparin, EDTA, citrate).
Mouse
96T
货号 | EK2047 |
---|---|
产品名称 | Mouse CLEC4F ELISA Kit |
产品描述 | For quantitative detection of mouse CLEC4F in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, R70-G548 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <300pg/ml |
检测范围 | 625pg/ml-40000pg/ml |
基因名称 | CLEC4F |
基因全名 | C-type lectin domain family 4, member f |
Uniprot ID | CLEC4F: Q8N1N0 |
指标别名 | C-type lectin domain family 4 member F; C-type lectin superfamily member 13; C-type lectin 13; CLEC4F; CLECSF13 |
背景资料 | C-type lectin domain family 4 member F is a protein that in humans is encoded by the CLEC4F gene. This gene is mapped to 6; 6 C3. CLEC4F is an 80 kDa, type II transmembrane glycoprotein member of the C-type lectin superfamily. Mature mouse CLEC4F consists of a 42 amino acid (aa) cytoplasmic domain, a 27 aa transmembrane segment, and a 479 aa extracellular domain that contains an extended stalk region plus one carbohydrate recognition domain. Within the ECD, mouse CLEC4F shares 48% and 79% aa sequence identity with human and rat CLEC4F, respectively. The stalk region of CLEC4F is a coiled coil domain that mediates homotrimer formation. CLEC4F is expressed on Kupffer cells in the liver, but not on macrophages in other tissues. CLEC4F preferentially binds galactose and Nacetylgalactosamine in a calcium-dependent manner. Its activity at neutral, but not at acidic pH, suggests a capacity to internalize and release ligands into the endosomal system. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2047-CAP | 96-well plate precoated with anti- mouse CLEC4F antibody | 1 |
EK2047-ST | Lyophilized recombinant mouse CLEC4F standard | 40ng/tube×2 |
EK2047-DA | Biotinylated anti- mouse CLEC4F antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 963 | 3961 | 15064 | 1024 | 4128 | 16938 |
Standard deviation | 46.22 | 225.78 | 692.94 | 52.22 | 243.55 | 1084.03 |
CV(%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
Concentration(pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
O.D. | 0.045 | 0.139 | 0.224 | 0.395 | 0.755 | 1.125 | 1.796 | 2.349 |
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