大家都在搜
手机验证
发货周期:现货
博士德生物
cell culture supernates, serum and plasma (heparin, EDTA).
Mouse
96T
货号 | EK1620 |
---|---|
产品名称 | Mouse TFF1 ELISA Kit |
产品描述 | For Quantitative Detection of mouse TFF1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, Q22-F87 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | Tff1 |
基因全名 | trefoil factor 1 |
Uniprot ID | Q08423 |
指标别名 | Trefoil factor 1|Protein pS2|Tff1|Bcei|Ps2 |
背景资料 | TFF1(Trefoil factor 1), also known as pS2, is a protein that in humans is encoded by the TFF1 gene. Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer, and affect healing of the epithelium. It is found that TFF1 in normal human urine inhibited the growth of calcium oxalate crystals. Urinary TFF1 showed an inhibitory potency similar to that of nephrocalcin, and inhibition was dose dependent and inhibited by TFF1 antisera, particularly by antisera directed to the TFF1 C terminus. Concentrations and relative amounts of TFF1 in the urine of patients with idiopathic calcium oxalate kidney stones were significantly less than those found in controls. This gene, which is expressed in the gastric mucosa, has also been studied because of its expression in human tumors. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. |
显色时间 | 20-25分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1620-CAP | 96-well plate precoated with anti- mouse TFF1 antibody | 1 |
EK1620-ST | Lyophilized recombinant mouse TFF1 standard | 10ng/tube×2 |
EK1620-DA | Biotinylated anti- mouse TFF1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 52 | 244 | 807 | 47 | 265 | 781 |
Standard deviation | 3.9 | 11.95 | 42.77 | 4.32 | 14.57 | 53.88 |
CV(%) | 7.5% | 4.9% | 5.3% | 9.2% | 5.5% | 6.9% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.095 | 0.220 | 0.305 | 0.462 | 0.780 | 1.196 | 1.657 | 2.031 |
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。