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发货周期:现货
博士德生物
cell culture supernates, serum and plasma (heparin, EDTA).
96T
货号 | EK1619 |
---|---|
产品名称 | Human NOTCH1 ELISA Kit |
产品描述 | For Quantitative Detection of human NOTCH1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, A19-Q526 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <12pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | NOTCH1 |
基因全名 | notch 1 |
Uniprot ID | P46531 |
指标别名 | Neurogenic locus notch homolog protein 1|Notch 1|hN1|Translocation-associated notch protein TAN-1|NOTCH1|TAN1 |
背景资料 | Notch proteins are single-pass transmembrane receptors that regulate cell fate decisions during development. The Notch family includes 4 receptors, NOTCH1, NOTCH2, NOTCH3, and NOTCH4, whose ligands include JAG1, JAG2, DLL1), DLL3, and DLL4. Notch homolog 1, translocation-associated (NOTCH1), is a human gene encoding a single-pass transmembrane receptor. It functions as a receptor for membrane bound ligands, and may play multiple roles during development. NOTCH1 may normally coordinates the process of somitogenesis, and the activated Notch 1 and Notch 3 promote differentiation of progenitor cells into astroglia. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1619-CAP | 96-well plate precoated with anti- human NOTCH1 antibody | 1 |
EK1619-ST | Lyophilized recombinant human NOTCH1 standard | 10ng/tube×2 |
EK1619-DA | Biotinylated anti- human NOTCH1 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 162 | 460 | 1757 | 166 | 428 | 1780 |
Standard deviation | 9.39 | 34.5 | 103.66 | 11.12 | 38.94 | 106.8 |
CV(%) | 5.8% | 7.5% | 5.9% | 6.7% | 9.1% | 6% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.014 | 0.093 | 0.154 | 0.268 | 0.473 | 0.822 | 1.388 | 1.924 |
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