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发货周期:现货
博士德生物
cell culture supernates, serum and plasma (heparin, EDTA).
96T
货号 | EK1644 |
---|---|
产品名称 | Human HAVCR2 ELISA Kit |
产品描述 | For Quantitative Detection of human TIM-3 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO,S22-G202 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | HAVCR2 |
基因全名 | hepatitis A virus cellular receptor 2 |
Uniprot ID | HAVCR2: Q8TDQ0 |
指标别名 | Hepatitis A virus cellular receptor 2; HAVcr-2; T-cell immunoglobulin and mucin domain-containing protein 3; TIMD-3; T-cell immunoglobulin mucin receptor 3; TIM-3; T-cell membrane protein 3; HAVCR2; TIM3; TIMD3 |
背景资料 | Hepatitis A virus cellular receptor 2 (HAVCR2), also known as T-cell immunoglobulin and mucin-domain containing-3 (TIM-3), is a protein that in humans is encoded by the HAVCR2 gene. The protein encoded by this gene belongs to the immunoglobulin superfamily, and TIM family of proteins. CD4-positive T helper lymphocytes can be divided into types 1 (Th1) and 2 (Th2) on the basis of their cytokine secretion patterns. Th1 cells are involved in cell-mediated immunity to intracellular pathogens and delayed-type hypersensitivity reactions, whereas, Th2 cells are involved in the control of extracellular helminthic infections and the promotion of atopic and allergic diseases. This protein is a Th1-specific cell surface protein that regulates macrophage activation, and inhibits Th1-mediated auto- and alloimmune responses, and promotes immunological tolerance. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1644-CAP | 96-well plate precoated with anti- human TIM-3 antibody | 1 |
EK1644-ST | Lyophilized recombinant human TIM-3 standard | 10ng/tube×2 |
EK1644-DA | Biotinylated anti- human TIM-3 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 51 | 224 | 977 | 50 | 216 | 884 |
Standard deviation | 2.44 | 10.08 | 68.39 | 2.55 | 10.36 | 74.25 |
CV(%) | 4.8% | 4.5% | 7% | 5.1% | 4.8% | 8.4% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.007 | 0.082 | 0.157 | 0.301 | 0.592 | 1.050 | 1.862 | 2.422 |
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