The Bosterbio OneStep ELISA kit is a solid phase direct ELISA sandwich kit. Instead of adding samples detection antibody and ABC-HRP separately, The OneStep ELISA kit allows the user to add standards, samples and controls to wells in one step, along with the incubation buffer. After a simple washing step, an enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, the substrate is added into each well. The enzyme catalyzes the substrate yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution. The intensity of color developed is directly proportional to the concentration of target protein in the samples. A standard curve is generated relating color intensity to the concentration of target protein.
Overview
Product Name
Human Free Prostate Specific Antigen (f-PSA) ELISA Kit
SKU/Catalog Number
EK7079
Description
Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit, tested with Serum and Plasma. Format: 96wells/kit, with removable strips.
Cite This Product
Human Free Prostate Specific Antigen (f-PSA) OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7079)
Validated Species
Human
Application
ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
Cross Reactivity
There is no detectable cross-reactivity.
Pack Size
96wells/kit, with removable strips.
Properties
Sensitivity
1 ng/ml *Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive.
Assay Range
1-25 ng/ml *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range.
Sample Type
Serum and Plasma
*The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it. **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide.
Storage
Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Kit Components
Description
Quantity
1. Microwells coated with Streptavidin
12x8x1
2. f-PSA Standard: 6 vials (ready to use)
0.5 ml
3. f-PSA Conjugate Reagent: 1 bottle (ready to use)