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发货周期:现货
博士德生物
cell culture supernates, serum, plasma (heparin, EDTA) and urine.
96T
货号 | EK1634 |
---|---|
产品名称 | Human Serpin A1/AAT ELISA Kit |
产品描述 | For quantitative detection of human Serpin A1 in cell culture supernates, serum, plasma (heparin, EDTA) and urine. |
免疫原 | NSO,E25-K418 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 1.56ng/ml-100ng/ml |
基因名称 | Serpin A1 |
基因全名 | serpin family A member 1 |
Uniprot ID | P01009 |
指标别名 | Alpha-1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1; Short peptide from AAT; SPAAT; SERPINA1; AAT; PI; PRO0684; PRO2209 |
背景资料 | Alpha-1-antitrypsinorα1-antitrypsin(A1AT,A1A, orAAT) is aproteinbelonging to theserpinsuperfamily. It is encoded in humans by theSERPINA1gene.Thegeneis located on the long arm of the fourteenth℃hromosome(14q32.1).The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.Like allserine protease inhibitors, A1AT has a characteristicsecondary structureofbeta sheetsandalpha helices.Mutationsin these areas can lead to non-functional proteins that canpolymeriseand accumulate in theliver(infantile hepatic cirrhosis). |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1634-CAP | 96-well plate precoated with anti- Human Serpin A1 antibody | 1 |
EK1634-ST | Lyophilized recombinant Human Serpin A1 standard | 100ng/tubex2 |
EK1634-DA | Biotinylated anti- Human Serpin A1 antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 2451 | 15117 | 38332 | 2323 | 15142 | 40979 |
Standard deviation | 144.6 | 1179.12 | 2529.91 | 157.96 | 1423.34 | 3114.4 |
CV(%) | 5.9% | 7.8% | 6.6% | 6.8% | 9.4% | 7.6% |
Concentration(ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
O.D. | 0.083 | 0.112 | 0.237 | 0.403 | 0.679 | 1.050 | 1.622 | 2.128 |
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