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发货周期:现货
博士德生物
cell culture supernates, serum and plasma (heparin, EDTA, citrate).
96T
货号 | EK2108 |
---|---|
产品名称 | Human ANTXR2 ELISA Kit |
产品描述 | For quantitative detection of human ANTXR2 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, Q34-G318 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | ANTXR2 |
基因全名 | ANTXR cell adhesion molecule 2 |
Uniprot ID | P58335 |
指标别名 | Anthrax toxin receptor 2; Capillary morphogenesis gene 2 protein; CMG-2; ANTXR2; CMG2 |
背景资料 | Anthrax toxin receptor 2is aproteinthat in humans is encoded by theANTXR2gene. It is mapped to 4q21.21. This gene encodes a receptor for anthrax toxin. The protein binds to collagen IV and laminin, suggesting that it may be involved in extracellular matrix adhesion. Mutations in this gene cause juvenile hyaline fibromatosis and infantile systemic hyalinosis. Multiple transcript variants encoding different isoforms have been found for this gene. |
显色时间 | 25-30分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2108-CAP | 96-well plate precoated with anti- human ANTXR2 antibody | 1 |
EK2108-ST | Lyophilized recombinant human ANTXR2 standard | 10ng/tube×2 |
EK2108-DA | Biotinylated anti- human ANTXR2 antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 189 | 1630 | 5138 | 181 | 1712 | 5150 |
Standard deviation | 13.6 | 91.28 | 339.1 | 13.75 | 111.28 | 427.45 |
CV(%) | 7.2% | 5.6% | 6.6% | 7.6% | 6.5% | 8.3% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.000 | 0.075 | 0.173 | 0.287 | 0.525 | 0.941 | 1.542 | 2.314 |
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