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文献和实验Antibody-Induced Opsonophagocytosis of Serogroup B Meningococci Measured by Flow Cytometry
meningococci, there seems to be a correlation between SBA activity and protection (1 ), whereas OP has been less well-studied. This function may have a supplementary or even major role in protection against group B meningococci.
Buffer and save the flowthrough.(12) Wash with 6 ml Buffer A, 6 ml Buffer B, and 6 ml Buffer A. Hold at room temperature for 30 minutes.(13) Wash with 6 ml Buffer B, 6 ml Buffer A, and 6 ml Buffer B. Wash with Storage Buffer and hold at 4°C.(14) To purify
this method will dilute the antibody by a factor of 2. Using a PD-10 column: a)Wash PD-10 column with 30mL of PBS. b) Add the 1mL FITC-Ig or Biotin Ig solution after the top of column is almost dry. Close stopcock to stop flow after the Antibody
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