PHOSPHO1 protein (His tag)

干货 | CUT and Tag 核心酶 Hyperactive PA/PG-Tn5 Transposase 大解密

CUT&Tag（Cleavage Under Targets and Tagmentation）是蛋白-DNA 互作的一大革新技术，它不需要使用甲醛交联以及免疫共沉淀，而是通过针对靶蛋白（如转录因子、染色质重塑蛋白）的抗体和 Protein A/G 的介导，使得与 Protein A/G 融合的 Tn5 酶(Tagmentase)在切割 DNA 片段的同时在序列两端加上测序接头，经 PCR 扩增后即可形成用于高通量测序的文库（见图 1 ）。CUT &Tag 技术具有细胞投入量低、信噪

Phos-tag Affinity Electrophoresis for Protein Kinase Profiling

Protein kinase profiling can provide a basis for understanding the molecular origins of diseases and, potentially, for developing tools for therapeutic intervention. It is therefore very important to develop advanced

Comparison of Protein Expression by Isotope-Coded Affinity Tag Labeling

Isotope-coded affinity tag (ICAT) labeling, in combination with mass spectrometry (MS), has been widely adopted as an effective method for comparing protein abundance levels. This chapter describes the ICAT labeling procedure in search