His-Tagged Protein Purificatio
n Kit Ni-Agarose His标签蛋白纯化试剂盒 蛋白纯化试剂 protein extraction 康为世纪- ￥398
- CW0009
- 2025年07月16日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存：
大量
- 规格：
100ug
| 目录号 | 规格 | 价格RMB | 品牌 |
| CW0009 | 2ml | 398.00 | CWBIO |
| CW0009A | 5ml | 598.00 | CWBIO |
| Ni-Agarose Resin for 6x His-Tagged Proteins Ni琼脂糖凝胶 |
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| 目录号 | 规格 | 价格RMB | 品牌 | |
| CW0010 | 2ml | 198.00 | CWBIO | |
| CW0010A | 10ml | 598.00 | CWBIO | |
| CW010B | 1ml | 118.00 | CWBIO | |
| 目录号 | 规格 | 价格RMB | 品牌 |
| CW0011 | 1ml | 498.00 | CWBIO |
| CW011A | 5ml | 1598.00 | CWBIO |
| 目录号 | 规格 | 价格RMB | 品牌 |
| CW0012 | 1ml | 598.00 | CWBIO |
| CW0012A | 5ml | 1998.00 | CWBIO |
| 目录号 | 规格 | 价格RMB | 品牌 |
| CW2009 | 1ml | 688.00 | CWBIO |
| 目录号 | 规格 | 价格RMB | 品牌 |
| CW2010 | 0.5ml | 968.00 | CWBIO |
| 目录号 | 规格 | 价格RMB | 品牌 |
| CW0190 | 2ml | 598.00 | CWBIO |
| CW0190A | 10ml | 1698.00 | CWBIO |
| 目录号 | 规格 | 价格RMB | 品牌 |
| CW0191 | 2ml | 498.00 | CWBIO |
| CW0191A | 10ml | 1598.00 | CWBIO |
| 目录号 | 规格 | 价格RMB | 品牌 |
| CW0192 | 2ml | 298.00 | CWBIO |
| CW0192A | 10ml | 898.00 | CWBIO |
| 目录号 | 规格 | 价格RMB | 品牌 |
| CW0193 | 2ml | 398.00 | CWBIO |
| CW0193A | 10ml | 998.00 | CWBIO |
| 目录号 | 规格 | 价格RMB | 品牌 |
| CW0194 | 2ml | 298.00 | CWBIO |
| CW0194A | 10ml | 898.00 | CWBIO |
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文献和实验Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
crude sonicated sample Dispense into 1.5 ml eppendorf tubes,spin 14k rpm,4℃,30 min Combine soluble fractions into 1 tube Take 30μl soluble fraction as post-sonication soluble sample Test solubility of target protein by SDS-PAGE/Coomassie Blue Stain
HIS-tagged protein purification
cells (from 500ml)in fliptop in 25 mls.UPB8.0,freeze lN2. 3)thaw at37℃ sonicate 30" high,freeze lN2,thaw. 4)spin RT 10' 15k ss34,respin in fresh tube 10' 15k ss34. 5)decant again to new tube,add 5ml 50% Ni-NTA resin,mix gently RT 30'. 6)spin,decant
6xHis-tagged protein purification using Qiagen Ni-NTA Column
)Centrifuge lysate at 10,000xg for 20’ at 40℃to pellet the cellular debris and save supernatant.Add 5μl 2xSB to 5μl Supernatant and store at 200℃for SDS-PAGE analysisBatch purification under Native ConditionsAdd 2.5 ml of the 50% Ni-NTA slurry to 10 ml cleared
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