Xenopus Laevis, Monkey, Fruit Fly, Dog, Chicken, C. Elegans, Arabidopsis Thaliana
应用范围
WB
抗体来源
Rabbit
免疫原
Synthetic dimethyl-peptide corresponding to human Histone H3.
交叉反应
No cross reactivity with other proteins.
抗体规格
100μL
产品详情
克隆
Polyclonal
产品形态
Liquid
内容
Each vial contains 50% glycerol and 0.09% sodium azide. *可以提供无载体抗体。
浓度
83 mg/ml by Refractometry
储存条件
At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time. Avoid repeated freezing and thawing.
Core component of nucleosome. Nucleosomes wrap andcompact DNA into chromatin, limiting DNA accessibility to thecellular machineries which require DNA as a template. Histonesthereby play a central role in transcription regulation, DNArepair, DNA replication and chromosomal stability. DNAaccessibility is regulated via a complex set of post-translationalmodifications of histones, also called histone code, andnucleosome remodeling.
[list_product_images]Western blot analysis of Histone H3 expression in HEK293 cell lysates (lane 1), HeLa cell lysates (lane 2), MCF-7 cell lysates (lane 3), Jurkat cell lysates (lane 4), A431 cell lysates (lane 5), A549 cell lysates (lane 6), LNCap cell lysates (lane 7), MOLT-4 cell lysates (lane 8), Ramos cell lysates (lane 9), Raji cell lysates (lane 10), A-172 cell lysates (lane 11) and NIH/3T3 cell lysates (lane 12). Histone 3 at 15 kDa was detected using rabbit anti-Histone H3 polyclonal antibody (Catalog # A12477) at 1:500. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).|Western blot analysis of Histone H3 expression in Raji cell lysates (lane 1) and HeLa cell lysates (lane 2). Histone 3 at 15.4 kDa was detected using rabbit anti-Histone H3 polyclonal antibody (Catalog # A12477) at 1:2000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).|Histone H3 was detected in paraffin-embedded sections of lung tissues using rabbit anti-Histone H3 polyclonal antibody (Catalog # A12477) at 1:100. Image provided courtesy of Andrew Elston, LifeSpan BioSciences, Inc.[/list_product_images]