Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
浓度
500 ug/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
MAP3K1(Mitogen-activated protein kinase kinase kinase 1), also known as MEKK1, MAPKKK1, MEK KINASE or MAP/ERK KINASE KINASE 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al.(1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse Map3k1 gene and the human MAP3K1 homolog. Using these allele-specific polymorphisms, they mapped the Map3k1 gene in an intersubspecific backcross to mouse chromosome 13. They mapped the human MAP3K1 gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al.(1998) showed that human MEKK1 activated JNK1(MAPK8) robustly and p38-alpha(MAPK14) less efficiently, but it had only a marginal effect on ERK2(MAPK1). MEKK1 directly and specifically interacted with JNKK1(MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 by MEKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al.(1998) concluded that JNKK1 is the preferred MEKK1 substrate.
研究类别
1. Juriloff, D. M., Harris, M. J., Mah, D. G. The open-eyelid mutation, lidgap-Gates, is an eight-exon deletion in the mouse Map3k1 gene. Genomics 85: 139-142, 2005.2. Vinik, B. S., Kay, E. S., Fiedorek, F. T., Jr. Mapping of the MEK kinase gene (Mekk) to mouse chromosome 13 and human chromosome 5. Mammalian Genome 6: 782-783, 1995.3. Xia, Y., Wu, Z., Su, B., Murray, B., Karin, M. JNKK1 organizes a MAP kinase module through specific and sequential interactions with upstream and downstream components mediated by its amino-terminal extension. Genes Dev. 12: 3369-3381, 1998.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.