A peptide derived from human CDH4. Immunogen sequence location: 731-780
内容
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
纯化方式
The antibody was purified from rabbit antiserum by affinity-chromatography using immunogen.
浓度
500 ug/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Cadherin-4 is a protein that in humans is encoded by the CDH4 gene. It is mapped to 20q13.33. This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In addition, a role in kidney and muscle development is indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in cotransfected cell lines. Previously thought to interact in an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. Three transcript variants encoding different isoforms have been found for this gene.
研究类别
1. Chalmers, I. J., Hofler, H., Atkinson, M. J. Mapping of a cadherin gene cluster to a region of chromosome 5 subject to frequent allelic loss in carcinoma. Genomics 57: 160-163, 1999.2. Suzuki, S., Sano, K., Tanihara, H. Diversity of the cadherin family: evidence for eight new cadherins in nervous tissue. Cell Regul. 2: 261-270, 1991.3. Tanihara, H., Sano, K., Heimark, R. L., St. John, T., Suzuki, S. Cloning of five human cadherins clarifies characteristic features of cadherin extracellular domain and provides further evidence for two structurally different types of cadherin. Cell Adhes. Commun. 2: 15-26, 1994.
User need to optimize the dilution ratio for this antibody
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CDH4 Antibody. The picture on the right is blocked with the synthesized peptide.|Immunofluorescence analysis of A549 cells, using CDH4 Antibody. The picture on the right is blocked with the synthesized peptide.[/list_product_images]