Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
浓度
500 ug/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene. The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. Additionally, this protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
研究类别
1. Apte, S. S.; Mattei, M.-G.; Olsen, B. R. : Mapping of human BAX gene to chromosome 19q13.3-q13.4 and isolation of a novel alternatively spliced transcript, BAX-delta. Genomics 26: 592-594, 1995.2. Guo, B.; Zhai, D.; Cabezas, E.; Welsh, K.; Nouraini, S.; Satterthwait, A. C.; Reed, J. C. : Humanin peptide suppresses apoptosis by interfering with Bax activation. Nature 423: 456-461, 2003.3. Oltvai, Z. N.; Milliman, C. L.; Korsmeyer, S. J. : Bcl-2 heterodimers in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 74: 609-619, 1993.4. Takeuchi, O.; Fisher, J.; Suh, H.; Harada, H.; Malynn, B. A.; Korsmeyer, S. J. : Essential role of BAX,BAK in B cell homeostasis and prevention of autoimmune disease. Proc. Nat. Acad. Sci. 102: 11272-11277, 2005.
Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT)Immunofluorescence (1-2ug/ml)
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[list_product_images]Formalin-fixed, paraffin-embedded Hodgkin's Lymphoma stained withBax Monoclonal Antibody (BAX/962).|SDS-PAGE Analysis Purified Bax Mouse MAb (Clone BAX/962). Confirmation of Integrity and Purity of Antibody|Flow Cytometric Analysis of Human Jurkat Cells using Bax Mouse MAb (Clone BAX/962) followed by Goat anti-mouse IgG-CF488 (Orange)|Confocal Immunofluorescence of Human Jurkat Cells A. Bax Mouse MAb (Clone BAX/962) B. Goat anti mouse CF488. labeled with CF488 (Green)|Formalin-fixed[/list_product_images]