12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
克隆性:
单克隆
适应物种:
Human,Rat,Rabbit
抗原来源:
Mouse
库存:
现货
供应商:
博士德生物
应用范围:
WB
浓度:
Concentration: 0.5-1μg/ml; Species: Human, rat, rabbit
规格:
50ul/100ul/150ul
规格:
50ul
产品价格:
¥860.0
规格:
100ul
产品价格:
¥1520.0
规格:
150ul
产品价格:
¥2280.0
产品概况
货号
BM1607
产品名称
ANTI-TPH1 Antibody
基因名
TPH1; TPH2
抗体来源
Mouse
克隆
Monoclonal (Clone: WH-3)
抗体亚型
Mouse IgG3
免疫原
Recombinant rabbit tryptophan hydroxylase.
内容
500ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA
纯化方式
Ascites
浓度
Concentration: 0.5-1μg/ml; Species: Human, rat, rabbit
产品形态
Lyophilized
保存条件
12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing.
背景资料
Tryptophan hydroxylase is the rate-limiting enzyme in the synthesis of serotonin(5-hydroxytryptamine, or 5HT). Tryptophan hydroxylase\atalyzes the biopterin-dependent monooxygenation of tryptophan to5-hydroxytryptophan(5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. Human tryptophan hydroxylase(TPH) is mapped to chromosome 11p15.3-p14 by in situ hybridization.
研究类别
1. Craig, S. P.; Boularand, S.; Darmon, M. C.; Mallet, J.; Craig, I. W. : Localization of human tryptophan hydroxylase (TPH) to chromosome 11p15.3-p14 by in situ hybridization. Cytogenet. Cell Genet. 56: 157-159, 1991. 2. Nielsen, D. A.; Dean, M.; Goldman, D. : Genetic mapping of the human tryptophan hydroxylase gene on chromosome 11, using an intronic conformational polymorphism. Am. J. Hum. Genet. 51: 1366-1371, 1992.
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0001-1) for IHC(P). *Blocking peptide 可以联系我们购买。
[list_product_images]Lane 1: Rat Brain Tissue LysateLane 2: Rat Brain Tissue Lysate|Figure 2. Western blot analysis of Tryptophan Hydroxylase using anti-Tryptophan Hydroxylase antibody (BM1607).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Caco-2 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Tryptophan Hydroxylase antigen affinity purified monoclonal antibody (Catalog # BM1607) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Tryptophan Hydroxylase at approximately 51KD. The expected band size for Tryptophan Hydroxylase is at 51KD.[/list_product_images]