E.coli-derived human KCNH2 recombinant protein (Position: R159-A223).
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
KCNH2, also known as HERG or KV11.1, encodes the pore-forming subunit of a rapidly activating-delayed rectifier potassium channel. It is mapped to 7q36.1. KCNH2 forms the major portion of one of the ion channel proteins (the 'rapid' delayed rectifier current (IKr)) that conducts potassium (K+) ions out of the muscle cells of the heart (cardiac myocytes), and this current is critical in correctly timing the return to the resting state (repolarization) of the cell membrane during the cardiac action potential. What’s more, KCNH2 channels show gating properties consistent with many of the outwardly rectifying potassium channels, but they also have an inactivation mechanism that attenuates efflux during depolarization.
研究类别
1. Gianulis, E. C., Trudeau, M. C. Rescue of aberrant gating by a genetically encoded PAS (Per-Arnt-Sim) domain in several long QT syndrome mutant human ether-a-go-go-related gene potassium channels. J. Biol. Chem. 286: 22160-22169, 2011.2. Hedley PL; Jorgensen P; Schlamowitz S; Wangari, Romilda et al. (2009). "The genetic basis of long QT and short QT syndromes: a mutation update". Human Mutation 30 (11): 1486–511.3. Thomas, P. J., Qu, B.-H., Pedersen, P. L. Defective protein folding as a basis of human disease. Trends Biochem. Sci. 20: 456-459, 1995.
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[list_product_images]Figure 1. Western blot analysis of KCNH2 using anti-KCNH2 antibody (A00781-1). Lane 1: human K562 whole cell lysatesLane 2: human U2OS whole cell lysates anti-KCNH2 antigen affinity purified polyclonal antibody (Catalog # A00781-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for KCNH2 at approximately 150KD. The expected band size for KCNH2 is at 127KD.[/list_product_images]