WB: 1:10,000-25,000. ICC/IF and IHC: 1:1,000-5,000.
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[list_product_images]Western blot analysis of different tissue lysates using rabbit pAb to NF-H, M05307-6, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord [4] mouse brain, and [5] mouse spinal cord lysate. Strong band at about 220kDa corresponds to the phosphorylated axonal form of the NF-H subunit. Smaller proteolytic fragments of NF-H are also detected with M05307-6 antibody.|Immunohistological analysis of a mouse hippocampus section stained with rabbit pAb to NF-H, M05307-6, dilution 1:2,000 in red, and costained with mouse mAb to myelin basic protein (MBP), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45?M, and free-floating sections were stained with above antibodies. The NF-H antibody labels a network of axons of different neurons, while the MBP antibody stains myelin sheath around these axons.[/list_product_images]