Recombinant construct containing the 2nd, 3rd and 4th Ki67 repeats of the human sequence (amino acids 1,111-1,490) expressed in and purified from E. coli.
内容
Affinity purified antibody at 1mg/mL in 50% PBS, 50% glycerol, 5mM azide
浓度
500 ug/ml
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
[list_product_images]Western blot analysis of equal amounts of cell lysates using mouse mAb to Ki67, M00254-2, dilution 1:2,000, in green: [1] protein standard (red), [2] rapidly dividing HeLa cell cultures, [3] rapidly dividing HEK293 cell cultures. Strong double bands above 250kDa correspond to the two major Ki67 isoforms of apparent molecular weight of 345kDa and 395kDa. Smaller proteolytic fragments of these isoforms are also invariably detected on the blot.|High magnification confocal immunofluorescence image of M00254-2 stained HeLa cells grown in tissue culture, in green. The nuclei of rapidly dividing cells have clumps of Ki-67 associated with nucleoli, while nearby quiescent cells express little or no Ki-67. Cells are counterstained with other in red which reveals the nuclear lamina, and DAPI reveals nuclear DNA in blue.[/list_product_images]