A peptide corresponding to the C-terminus of alpha -Tubulin
内容
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
浓度
500 ug/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Alpha-tubulin(b-alpha-1) mRNA is expressed only in brain with a molecular weight of about 55,000. The 3-prime UTR of b-alpha-1 is more than 80% homologous to the UTR of the rat brain alpha-tubulin gene, IL-alpha-T1. B-alpha-1 encodes a predicted 451-amino acid protein that is 100% identical to the rat homolog and differs by only 2 and 3 amino acids from the pig and chicken homologs, respectively.
研究类别
1. Cowan, N. J.; Dobner, P. R.; Fuchs, E. V.; Cleveland, D. W. : Expression of human alpha-tubulin genes: interspecies conservation of 3-prime untranslated regions. Molec. Cell. Biol. 3: 1738-1745, 1983. 2. Hall, J. L.; Cowan, N. J. : Structural features and restricted expression of a human alpha-tubulin gene. Nucleic Acids Res. 13: 207-223, 1985.
[list_product_images]Figure 1. Western Blotting resultWestern blot of A431 cells, using Clone RM113 at 1/1000 dilution. A-Tubulin band showed at the predicted MW (~50 kDa).|Figure 2. ICC resultImmunocytochemical staining of HeLa cells, using Anti-Tubulin Clone RM113 at 1/200 dilution (red). Nuclei have been labeled with DAPI (blue).[/list_product_images]