[list_product_images]Figure 1. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue section using anti-human Ig lambda light chain rabbit monoclonal antibody (Clone RM127).|Figure 2. ELISA result showing specificity
ELISA of human immunoglobulins shows RM127 reacted to the lambda light chain of human immunoglobulins. No cross reactivity with the kappa light chain, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL or 50 ng/mL of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.|Figure 3. ELISA result showing specificity
A titer ELISA using RM127. The plate was coated with different amounts of human IgG3?. A serial dilution of RM127 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.|Figure 4. ELISA result showing specificity
ELISA showing RM127 does not react to monkey IgG. The plate was coated with Rhesus monkey IgG. A serial dilution of RM127 and a monkey IgG binding antibody (positive control) was used as the detection antibody.[/list_product_images]