A peptide corresponding to the C-terminus of human Calponin-1
内容
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
浓度
500 ug/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
Calponin is a basic 34-kD protein first isolated from chicken gizzard and bovine aorta. This gene is mapped to 19p13.2. It is specifically expressed in smooth muscle and binds calmodulin, actin, and tropomyosin. It is able to inhibit the ATPase activity of myosin and is though to play a role in smooth muscle contraction. Calponin is a smooth muscle specific, actin-, tropomyosin- and calmodulin-binding protein though to be involved in some way in the regulation or modulation of contraction.
研究类别
1. Miano, J. M., Krahe, R., Garcia, E., Elliott, J. M., Olson, E. N. Expression, genomic structure and high resolution mapping to 19p13.2 of the human smooth muscle cell calponin gene. Gene 197: 215-224, 1997.2. Miano, J. M., Thomas, S., Disteche, C. M. Expression and chromosomal mapping of the mouse smooth muscle calponin gene. Mammalian Genome 12: 187-191, 2001.3. Strasser, P., Gimona, M., Moessler, H., Herzog, M., Small, J. V. Mammalian calponin: identification and expression of genetic variants. FEBS Lett. 330: 13-18, 1993.
Western blot(WB): 1:1000-1:2000 dilution;Immunocytochemistry (ICC): 1:100-1:200.
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[list_product_images]Figure 1. Western Blotting result
Western Blot analysis of mouse heart tissue lysates, using anti-Calponin-1 rabbit monoclonal antibody (Clone RM262), shows Calponin-1 expression in mouse heart tissue.|Figure 2. ICC result
Immunocytochemistry of HeLa cells, using Calponin-1 Rabbit Monoclonal Antibody (Clone RM262) (red). Actin filaments have been labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).[/list_product_images]