A peptide corresponding to the C-terminus of human Vimentin.
内容
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
浓度
500 ug/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
VIM(vimentin) is also known as HEL113 or CTRCT30. This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.
研究类别
1. Besarani D, et al. Role of anti-vimentin antibodies in renal transplantation. Transplantation, 2014 Jul 15.2. Kinloch AJ, et al. Vimentin is a dominant target of in situ humoral immunity in human lupus tubulointerstitial nephritis. Arthritis Rheumatol, 2014 Dec.3. Shirahata A, et al. Serum vimentin methylation as a potential marker for colorectal cancer. Anticancer Res, 2014 Aug.
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
*最佳稀释度需要用户自己调试,此处数据仅供参考。
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[list_product_images]Figure 1. Western Blotting result
Western Blot of HeLa lysates using anti-Vimentin rabbit monoclonal antibody (clone RM289) at a 1:400 dilution.|Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue sections using anti-Vimentin rabbit monoclonal antibody (clone RM289) at a 1:200 dilution.[/list_product_images]