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Monoclonal(Clone:AC-15)
human, mouse, rat, pig, monkey, rabbit, chicken
发货周期:现货
9999
博士德生物
WB, IHC
100μl/200μl/1ml
规格: | 100μl | 产品价格: | ¥880.0 |
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规格: | 200μl | 产品价格: | ¥1680.0 |
规格: | 1ml | 产品价格: | ¥6800.0 |
货号 | BM0627 |
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产品名称 | Anti-β-Actin Antibody |
基因名 | ACTB |
抗体来源 | mouse |
克隆 | Monoclonal(Clone:AC-15) |
抗体亚型 | Mouse IgG1 |
分子量 | 42KD(不适用于心肌样本的检测) |
免疫原 | slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. |
内容 | 200ug/ml antibody with PBS ,0.02% NaN3 , 1mg BSA |
纯化方式 | Ascites |
浓度 | 200ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia. |
Uniprot ID | ACTB: P60709 |
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot (WB): | 1:5000-20000 |
Immunohistochemistry in paraffin section (IHC): | 1:50-400 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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