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Monoclonal
human, mouse, rat
发货周期:现货
9999
博士德生物
WB, ICC/IF, FCM
100μl/200μl/1ml
规格: | 100μl | 产品价格: | ¥880.0 |
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规格: | 200μl | 产品价格: | ¥1680.0 |
规格: | 1ml | 产品价格: | ¥6800.0 |
货号 | M05613-4 |
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产品名称 | Anti-β-tubulin Antibody (monoclonal, 2E11) |
基因名 | TUBB |
抗体来源 | Mouse |
克隆 | Monoclonal(Clone:2E11) |
抗体亚型 | Mouse IgG2a |
分子量 | 55KD |
免疫原 | A synthetic peptide corresponding to a sequence at the C-terminus of human Beta Tubulin (383-412aa EQFTAMFRRKAFLHWYTGEGMDEMEFTEAE), identical to the related mouse and rat sequences. |
内容 | 500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500 ug/ml |
产品形态 | Liquid |
保存条件 | 12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
背景资料 | Tubulin beta chain is a protein that in humans is encoded by the TUBB gene. This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia,complex,with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1,6,7,8,9,and 13. |
Uniprot ID | TUBB: P07437 |
推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC. |
博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
应用 | 稀释度* |
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Western blot(WB): | 1:500-2000 |
Immunocytochemistry in fixed cells: | 1:50-400 |
Flow cytometry (FCM): | 1-3 μg/1x106 cells |
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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