E.coli-derived human CD79b recombinant protein (Position: A29-E229). Human CD79b shares 70% amino acid (aa) sequence identity with mouse CD79b.
内容
500 ug/ml antibody with PBS ,0.02% NaN3 , 1 mg BSA and 50% glycerol.
纯化方式
protein G purified.
浓度
500 ug/ml
产品形态
Liquid
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
CD79b molecule,immunoglobulin-associated beta,also known as CD79B (Cluster of Differentiation 79B),is a human gene. By fluorescence in situ hybridization,It is mapped to 17q23.3. The CD79B protein together with the related CD79A protein,forms a dimer associated with membrane bound immunoglobulin in B-cells,thus forming the B-cell antigen receptor (BCR) which is a multimeric complex that includes the antigen-specific component,surface immunoglobulin (Ig). CD79b also can enhances phosphorylation of CD79A,possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.
*最佳稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
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[list_product_images]Figure 1. Western blot analysis of anti- CD79B antibody (M01399-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,?
Lane 2: human Raji whole cell lysates,?Use mouse anti- CD79B 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for CD79B at approximately 35KD. The expected band size for CD79B is at 26KD.|Figure 2.IHC analysis using anti-CD79B antibody (M01399-1). detected in paraffin-embedded section of human tonsil cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.|Figure 3.IHC analysis using anti-CD79B antibody (M01399-1). detected in paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.[/list_product_images]