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文献和实验10X TBE BUFFER 3 Liters 324 g Tris base 165g Boric acid 120mls 0.5M EDTA (pH 8.0) autoclave for 20 min 2 Liters 216g Tris base 110g Boric acid 80mls 0.5M EDTA (pH 8.0) autoclave
Mag-Bind Blood DNA Maximum Yield Protocol (7ml-10ml)
containing the Mag-Bind particles from the magnetic separation device. Add 10 ml SPM Buffer to each sample. 13. Resuspend the Mag-Bind particles pellet by vortexing. Incubate 3 minutes at room temperature. Complete resuspension of the Mag- Bind
SQ Blood DNA Maxi Protocol for 4-10 ml whole blood
20-25 times. Incubate the mixture at 37°C for approximately 10 minutes. 6. Cool the sample to room temperature. Add 1/3 Volume of Buffer PCP to the cell lysate. Vortex vigorously at high speed for 30 seconds to mix. Some protein clumps
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