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文献和实验/translational regulation of target genes. These systems consist of “reporter” genes, such as lacZ, blaM, phoA, gfp, luxA , or cat , lacking either their own transcriptional or translational signals. The reporters are embedded within transposons in such a way
10 Targeting in Myocardial Infarction
. Therefore, the availability of a target-specific delivery system should increase the efficacy of diagnosis and therapy. Furthermore, the existence of such a targeted delivery system may pave the way for the use of new pharmaceuticals that by themselves can be harmful
TOP10 chemically competent cells
Overview This protocol is a variant of the Hanahan protocol [1] using CCMB80 buffer for DH10B, TOP10 and MachI strains. It builds on Example 2 of the Bloom05 patent as well. This protocol has been tested on TOP10, MachI and BL21(DE3) cells
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