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文献和实验Quantitation of Protein: Coomassie Bright Blue Dye - binding Method (Bradford Method)
with Coomassie bright blue Dye - binding method. 2. Try to compare the advantages and disadvantages of Bicinchoninic Acid Method with Folin - phenol Reagent Method. 3. What is the function of adding sulphuric acid and K 2 SO 4 -CUSO
soluble in the color reagent).The Bradford reagent should be a light brown in color.Filtration may have to be repeated to rid the reagent of blue components.The Bio-Rad concentrate is expensive,but the lots of dye used have apparently been screened
The bradford dye-binding assay is a colorimetric assay for measuring total protein concentration. It involves the binding of Coomassie Brilliant blue to protein. There is no interference from cations nor from carbohydrates
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