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文献和实验【转帖】2-D Clean-Up Kit 双向凝胶电泳--蛋白纯化
System (GE Healthcare). After separation in the first dimension, the strip is run on the second dimension and the gels are either commassie or silver stained. The contents of the kit are sufficient for 50 purifications. The kit is stable
Sequencing Reaction Cleanup in 384 Well Sequencing Reaction Plates
to the viper plates using the V-prep protocol with mixing. 2.Seal with a Silicone Sealing Mat. Stacking no higher than four plates, centrifuge at 3200 rpm, 4 ℃, for 30 min in the Beckman CS-6R tabletop centrifuge OR 2400 rpm, 4 ℃, for 30 min on the Jouan KR
Objective: Mini-Prep procedure is used to isolate small plasmid DNA from bacteria while limiting contaminating proteins and genomic DNA. The plasmid quality is acceptable for restriction analysis, sequencing, cloning, or other purposes
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