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- 2025年07月09日
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文献和实验DNA Recovery With Low Melt Agarose
. Be sure to overlay the gel with buffer before pulling out the comb, to prevent damage to the wells 3.Run gel in cold room, 100V 4.Cut out fragment of interest with clean razor blade and remove all excess agarose from the DNA. 5.Use long wave UV
Agarose-Formaldehyde Electrophoresis
M formaldehyde and 1 x MOPS, respectively. Example: For 50ml of a 1% agaro se gel, melt 0.5g agarose in 37ml H2 O, cool to hand hot, add 5ml 10 x MOPS buffer and 8.75ml 40% formaldehyde. Electrophoresis is in 1 x MOPS, 2.2M formaldehyde. RNA samples
ELECTROPHORESIS OF DNA IN AGAROSE GELS
ELECTROPHORESIS OF DNA IN AGAROSE GELS A). AGAROSE CONCENTRATIONS: Use 0.8% agarose (w/v) for high molecular weight DNA
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