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文献和实验1.After electrophoresis of protein gel,transfer gel to round staining tray.Add ~200 ml protein gel stain. 2.Microwave for ~45 sec until the solution just starts to boil.Incubate at room temp with gentle shaking for 10-15 min.Heating allows the gel
Protocol Protein Gel Staining Using Coomassie Blue Protein Stain
of SimplyBlue™ Coomassie protein stain and microwave on high for 45 seconds to 1 minute until the solution almost boils Shake the gel on a shaker for 10 minutes Wash the gel in 100 ml of ultrapure water for 10 minutes on a shaker Add 20 ml
15 g 15 g H20 10ml 7.0 ml Microwave ~10 seconds and stir until dissolved Degas 1-2 min Add 0.3 ml 10% ammonium persulfate Add 35 microliters TEMED. Pour Gel
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