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文献和实验Sephadex G-50 spun column purification
Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing
because it is often the buffer used during the first aldehyde fixation. 5) Post fix in 2% osmium tetroxide in 100 mM cacodylate buffer (1 hr on ice). Take care with the osmium tetroxide, even the fumes are dangerous. 6). Wash in water (3 x 5'). 7). Wash in 50 mM
Sephadex G-50 spun column purification
Sephadex G-50 spun column purification Spin column purification can be used to change buffers without a concomitant change in solution volume, to remove protein contaminants or to purify plasmid DNA suitable for sequencing. 1) Plug a 1
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