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- Bio-Rad Laboratories
- MSP9661
- 2025年07月14日
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文献和实验96 Well Mating Protocol platewise
on the XGAL plate 2. Streak recipient plasmid (pMSCV) on LB Spec50 CB100 0.2% glucose OR YEGlu spec50 cb100) Grow at 30oC overnight Protocol 1. Inoculate 5 ml single colony culture of recipient, and a 1:100 donor culture in 500 ul/well 96 well culture box
96 Well Mating Protocol platewise
+Spec50+CB100+0.2% glucose OR YEGlu+spec50+cb100) Grow at 30oC overnight Protocol1. Inoculate 5 ml single colony culture of recipient, and a 1:100 donor culture in 500 ul/well 96 well culture box. Grow recipient at 30oC and donor at 37℃, shaking
96-well RNA In Situ Hybridization Protocol
Noordermeer and Casey KopczynskiI.PREPARATION OF EMBRYOS1. Embryos were collected for 24 hours and washed 3X with 0.02% Triton X (Tx)(15ml for up to 2ml embryos in a 50ml falcon tube).2. Dechorionate 5 min. in 50% bleach.3. Wash 3X with 0.02% Tx.4. Fill tube
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