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文献和实验4kOARRBoVVdV0du4Vtqi/f6tUqYIq26tIr4TAuH7dOly5dBnHbWyk45k6FDF06rRpMDIyitYy9pcjh4+AB2k+BEsVCrbGhQlkZgIsfGTm3U/i2n2ebMfe90CZuV1hSmcnUd9MqjDuOBiVVk3Fpa0X8bVhZyQ9qoA3zq06gCHlB6A42YH4OOzB0oPu0Kg7DWb50taH+e/fv2FDX1S7d1jh7v0HaEi/moebj0U9MiYVNhXxlSZNm0vCh7BJmEZfcqdOn6R
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
type of interest, grown under appropriate cell culture conditions with appropriate medium Cells, bacterial, competent, high-efficiency ( 5 x 109 cfu/µg DNA) XL10-Gold ultracompetent cells (Stratagene) have produced optimal results.
100x Pen-Strep (P4333 or P0781) • 5 ml 100x sodium pyruvate (S8636 or P4562) • 5 ml beta-mercaptoethanol Stock solution = 3.5 µl diluted to 5 ml neat beta-maerpactoethanol (M7522) with tissue culture grade water (W3500) — make
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