- ￥675
- 国产知名品牌
- E1003
- 北京
- 2025年07月12日
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- 保存条件：
4ºC
- 供应商：
无锡柏欧美地生物
- 库存：
大量
- 保质期：
24个月
- 规格：
250次
| 甘油三酯(血液)酶法测定试剂盒 (Triglyceride GPO-POD assay Kit，E1003) | ||||
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| 描述：甘油三酯由甘油与脂肪酸酯酯化而成，是血脂的主要成分。高甘油三酯和高胆固醇血症是临床最常见的高脂血症，与多种疾病有关。试剂盒采用甘油磷酸氧化酶法与经典GPO Trinder酶学反应测定甘油三酯含量，符合世界卫生组织(WHO)、美国FDA、中国《全国临床检验操作规程》规定的甘油三酯临床检验标准。可以测定血液、食品饮料甘油三酯含量，灵敏度高，线性范围20~2000 µmol/L。 测定原理：(1) 脂肪酶分解血清中甘油三酯为甘油与脂肪酸。(2) 在ATP存在下，甘油激酶将甘油磷酸化生成3-磷酸甘油。(3) 后者被磷酸甘油氧化酶氧化生成磷酸二羟丙酮和过氧化氢。(4) 过氧化氢与过氧化物酶、4-氨基比林进行显色反应，生成有色苯醌亚胺，在500 nm (480~550 nm)处吸光度值与甘油三酯浓度成正比。 用途：测定血液、食品饮料的甘油三酯含量。组织细胞内甘油三酯测定请使用E1013。 组成 (250次微板测定或125次1 ml比色杯测定) (1) R1试剂 40 ml (2) R2试剂 10 ml (3) 4 mM甘油标准品1 ml 4 ºC避光储存3月。 所需设备：721、722型可见光分光光度计、酶标仪、生化分析仪。96孔微板或比色杯光径1cm。最佳工作波长550nm，如无此波长建议优先选用570nm、次选530、490nm。 部分发表论文： Zhang Xuequn, Yang Juntao, Guo Yuanbiao, Ye Hua, Yu Chaohui, Xu Chengfu, Xu Lei, Wu Songfeng, Sun Wei, Wei Hangdong, Gao Xue, Zhu Yunping, Qian Xiaohong, Jiang Ying, Li Youming, He Fuchu, 2009. Functional proteomic analysis of nonalcoholic fatty liver disease in rat models: Enoyl–coenzyme a hydratase down-regulation exacerbates hepatic steatosis. Hepatology, 51(4): 1190-1199. IF: 10.88 Luo Zhidan, Ma Liqun, Zhao Zhigang, He Hongbo, Yang Dachun, Feng Xiaoli, Ma Shuangtao, Chen Xiaoping, Zhu Tianqi, Cao Tingbing, Liu Daoyan, Nilius Bernd, Huang Yu, Yan Zhencheng, Zhu Zhiming, 2011. TRPV1 activation improves exercise endurance and energy metabolism through PGC-1α upregulation in mice. Cell Research, doi:10.1038/cr.2011.205. IF: 9.416 Pang Shanshan, Tang Haiqing, Zhuo Shu, Qin Zang Ying, Le Yingying, 2010. Regulation of Fasting Fuel Metabolism by Toll-Like Receptor 4. Diabetes, 59(12): 3041-3048. IF: 8.889 Ding Yunfeng, Yang Li, Zhang Shuyan, Wang Yang, Du Yalan, Pu Jing, Peng Gong, Chen Yong, Zhang Huina, Yu Jinhai, Hang Haiying, Wu Peng, Yang Fuquan, Yang Hongyuan, Steinbuchel Alexander, Liu Pingsheng, 2011. Identification of the major functional proteins of Prokaryotic lipid droplets. The Journal of Lipid Research, doi:10.1194/jlr.M021899. IF: 6.114 Zhao Zhigang, Luo Zhidan, Wang Peijian, Sun Jing, Yu Hao, Cao Tingbing, Ni Yinxing, Chen Jing, Yan Zhencheng, Liu Daoyan, Zhu Zhiming, 2011. Rosiglitazone Restores Endothelial Dysfunction in a Rat Model of Metabolic Syndrome through PPARγ- and PPARδ-Dependent Phosphorylation of Akt and eNOS. PPAR Research, doi:10.1155/2011/291656. IF: 2.726 Deng Jiagang, Li Chunyang, Wang Hailian, Hao Erwei, Du Zhengcai, Bao Chuanhong, Lv Jianzhen, Wang Yi, 2011. Amygdalin mediates relieved atherosclerosis in apolipoprotein E deficient mice through the induction of regulatory T cells. Biochemical and Biophysical Research Communications, 411(3): 523–529. IF: 2.593 Zhai, W., Xu, C., Ling, Y., Liu, S., Deng, J., Qi, Y., Londos, C., Xu, G., 2010. Increased Lipolysis in Adipose Tissues is Associated with Elevation of Systemic Free Fatty Acids and Insulin Resistance in Perilipin Null Mice, Hormone Metabolic Research, 42: 247-253. IF: 2.414 Yang Zhi, Yin Ji-Ye, Gong Zhi-Cheng, Huang Qiong, Chen Hao, Zhang Wei, Zhou Hong-Hao, Liu Zhao-Qian, 2009. Evidence for an effect of clozapine on the regulation of fat-cell derived factors. Clinica Chimica Acta, 408(1–2): 98-104. IF: 2.388 He Yong-Han, He Ying, Liao Xi-Lu, Niu Yu-Cun, Wang Guan, Zhao Chen, Wang Liang, Tian Ming-Jie, Li Ying, Sun Chang-Hao, 2012. The calcium-sensing receptor promotes adipocyte differentiation and adipogenesis through PPARγ pathway. Molecular and Cellular Biochemistry, 361(1-2): 321-328. IF: 2.167 Ju Dapeng, He Jingjing, Zhao Lili, Zheng Xueli, Yang Gongshe, 2011. Estrogen related receptor α-induced adipogenesis is PGC-1β-dependent. Molecular Biology Reports, 39(3): 3343-3354, DOI: 10.1007/s11033-011-1104-8. IF: 1.875 |
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甘油三酯(血液)酶法测定试剂盒
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