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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
嵘崴达
- 库存：
需确认
- 克隆性：
单克隆
- 保质期：
见产品外包装
- 抗体英文名：
Anti-RNA Polymerase II Antibody, clone ARNA-3
- 亚型：
IgG1
- 保存条件：
见产品外包装
- 规格：
100 μL
Anti-RNA Polymerase II Antibody, clone ARNA-3
ascites fluid, clone ARNA-3, Chemicon®
一般描述
DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; UniProt P04052; also known as DNA-directed RNA polymerase III largest subunit, RNA polymerase II subunit B1) is encoded by the RpII215 (also known as CG1554) gene (Gene ID 32100) in drosophila melanogaster (fruit fly). RNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
特异性
ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II in biological materials. Reactive polypeptides after SDS-PAGE: 200 kDa band of nonphosphorylated and 240 kDa band of phosphorylated Pol IIA.
Broad species cross-reactivity expected due to sequence homology.
免疫原
Epitope: Amino acids 794-822
Purified Drosophila RNA polymerase II
应用
Detect RNA Polymerase II with Anti-RNA Polymerase II Antibody, clone ARNA-3 (Mouse Monoclonal Antibody), that has been shown to work in WB & IHC.
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文献和实验An RNA Polymerase II In Vitro Transcription System
A great deal of attention in recent years has been focused on the mechanisms governing transcription. Repression and/or activation of specific genes, or sets of genes, represents a key regulatory step in such diverse processes as cell growth
Profiling RNA Polymerase II Using the Fast Chromatin Immunoprecipitation Method
polymerase II (Pol II) chromatin immunoprecipitation (ChIP) is a simpler alternative to determine the transcription rate of genes. Moreover, this approach provides more information about the transcriptional regulation of a gene than nuclear run-on. The power
mRNA Amplification with T7 RNA Polymerase
Materials MessageAmp II aRNA Amplication Kit (Cat #1751, Ambion) 100% Ethanol RNA samples (e.g. 1 µg RNA per sample) Procedure Reverse transcription Verify that EtOH (24 mL) has been added to the Wash Buffer. Turn
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