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文献和实验Peroxidase Conjugation by Periodate Method
Reagents Horseradish peroxidase Sodium periodate, 0.1M, freshly made Ethylene glycol Sephadex G-25 Sodium acetate buffer, 0.001M, pH 4.2 IgG Carbonate:Bicarbonate Buffer, 1.0 M, pH 9.5 Sodium tetraborate (4 mg/ml) freshly
Periodate Treatment of Fixed Cell Plates
1. Reagents Sodium Borohydride (NaBH4) - 1 mg/ml Carbonate:Bicarbonate Buffer, pH 9.6 Sodium Periodate (NaIO4) - 1 mM, 2.14 mg/10 ml Acetate Buffer 10mM Acetate Buffer, pH 4.5 0.82 g sodium acetate in 90 ml diH2O. Adjust to pH
Peroxidase Conjugation by Periodate Method
Reagents Horseradish peroxidase Sodium periodate, 0.1M, freshly made Ethylene glycol Sephadex G-25 Sodium
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