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- ProteoChem
- LC6334-1L
- 2025年07月14日
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文献和实验In-gel digestion of proteins for peptide fingerprint mapping
~3 extractions. For the first extraction, add 2 volumes of water and vortex for 10 min. For subsequent extractions, add 5% formic acid/50% acetonitrile. Use gel-loading tips to remove the peptide solution after each extraction and collect in a siliconized tube
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
L ) Spin down liquid and transfer the 'basic extracted' peptide containing solution to the same tube as the 'digest solution' Add 40-50 µl of 5% formic acid (to adjust the pH) Incubate for 15 min at 37°C while shaking Spin
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
solution to the same tube as the ''digest solution'' Add 40-50 µl of 5% formic acid (to adjust the pH) Incubate for 15 min at 37°C while shaking Spin down and add 100 % Acetonitrile (1-2 times equal the volume of the gel, 80µl)
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