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- 2025年07月14日
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文献和实验In-gel digestion of proteins for peptide fingerprint mapping
the final volume of the extracts to ~10 m l in a vacuum centrifuge and add 5 m l 5% Formic acid/ 50% acetonitrile. 10. Store the recovered peptides at -20 °C in slick tubes. References Fenselau, C. 1997. MALDI-MS and strategies
蛋白质和多肽MALDI-MS分析方法 General Method for MALDI-MS Analysis of Proteins and Peptides
Reagents Calibration standards (1-10 pmol/µl in 60% acetonitrile containing 0.1% [v/v] TFA) To obtain the best interpretation of MALDI-TOF-MS data, standards of known molecular mass close
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
L ) Spin down liquid and transfer the 'basic extracted' peptide containing solution to the same tube as the 'digest solution' Add 40-50 µl of 5% formic acid (to adjust the pH) Incubate for 15 min at 37°C while shaking Spin
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