相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Phosphodiesterase I from Crotalus adamanteus venom;5′-Exonuclease;Oligonucleate 5′-nucleotidohydrolase
- 保质期:
2年
- 供应商:
上海联迈生物工程有限公司
- 保存条件:
RT
- 规格:
瓶
| [产品编号] | LM1195M |
| [CAS号] | 9025-82-5 |
| [规格] | BR,20u/mg |
| [包装] | 100U |
| [到货期] | 2-3天 |
英文名称:Phosphodiesterase I from Crotalus adamanteus venom;5′-Exonuclease;Oligonucleate 5′-nucleotidohydrolase
其他名称:磷酸二脂酶;磷酸二酯酶
CAS号:9025-82-5
级别:BR
Specific activity:≥20u/mg protein
活力定义:One Unit hydrolyzes one micromole of p-nitrophenyl thymidine-5-phosphate per minute at 25℃, pH 8.9.
产品描述:Venom exonuclease (Phosphodiesterase I) successively hydrolyzes 5'-mononucleotides from 3'-OH-terminated ribo- and deoxyribo-oligonucleotides. The enzyme has an optimal pH range of 9.8-10.4 and a molecular weight of 115 kDa. Phosphodiesterase is inhibited by reducing agents such as glutathione, cysteine and ascorbic acids. It is completely inhibited by 5mM EDTA while ATP, ADP and AMP are partial inhibitors. The enzyme has an absolute requirement for Mg2+
性状(以下信息仅供参考):白色或类白色粉末
用途:本品仅供科研,不得用于其它用途。(以下用途仅供参考)用于序列核酸的研究
保存:-20℃
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验() #交换 x 轴和 y 轴> p + ylim(0,15) #如果想截取某一段可以用此命令设置值域。> p + scale_x_continuous(breaks =seq(18,34,50) ) #设置刻度线位置> p + theme(axis.text.x = element_text(angle = 90,family = "Times",face = "italic",colour = "darkred",size=rel(0.9))) # angle = 90 设置字体角度, family
IMMUNOHISTOCHEMISTRY ON SCHNEIDER LINE 2 CELLS
in the dark; wash 3-5X in PBS (1-20' total); mount in Citifluor. b)Vectastain: use biotinylated secondary; DAB-peroxidase substrate; mount in glycerol. Fix 5ml: 2.5% paraformaldehyde 0.78ml 16% stock 100mM NaHPO4 0.5ml 1M NaHPO4, pH 7.2-7.4 3.82ml
104dpm, we recur: 14.91 x 104 ---------------------- x 100% = 82.3% incorporation 18.12 x 104 0.823 x 22ng = 18.1ng DNA synthesized Thus, the specific activity is: 14.91 x 104dpm x 20 x 50
技术资料暂无技术资料 索取技术资料







