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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
5(6)-FAM, SE [5-(and-6)-Carboxyfluorescein, succinimidyl ester]
- 库存:
999
- 供应商:
博奥派克
- 规格:
100 mg
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文献和实验LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
Day 0 and 1 Materials 1.0.5 mM ATP,0.2 mM CaCl2,1 mM PIPES,pH 6.8 at 4℃,250 ml for day 0. 2.0.5 mM ATP,0.2 mM CaCl2,70 mM KCl,150 mM PIPES,pH 7.0,1 ml. 3.16 mM ATP,0.2 mM CaCl2,5 mM DTT,5 mM lysine,10 mM glutamic acid,20 mM Tris-HCl,pH 8.0,10 ml
that must be examined lack cytological detail. On the other hand, 5, 6 carboxy 2′,7′ dichloro-fluorescein diacetate succinimidyl ester (CCSFE), a carboxyfluorescein derivative that resists bleaching (5 ) better than the monochloro-form (1 ), is a stable cytoplasmic
how two-photon microscopy and carboxyfluorescein succinimidyl ester (CFSE or CFDA SE) labeling of T cells coupled with flow cytometry can be used as tools to assess chemokine-mediated regulation of T cell proliferation, activation, and migration.
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