- ￥6188
- Cytoskeleton
- CS-GE04
- 2025年07月12日
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文献和实验HIS-tagged protein purification
John Mundy,Institute of Molecular Biology,Copenhagen,Denmark 1 liter cell prep 1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about 1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze at 70℃ 2)resuspend
Proteins are composed of functional units, or domains, that can be found alone or in combination with other domains. Analysis of protein domain architectures and the movement of protein domains within and across different genomes provide
Domains are considered to be the building blocks of protein structures. A protein can contain a single domain or multiple domains, each one typically associated with a specific function. The combination of domains determines the function
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