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- Cytoskeleton
- BK030
- 2025年07月13日
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文献和实验因为自己实验后期需要使用这方面的技术,所以最近在查阅这方面的资料,想拿出来和大家一起分享.后期会把更详细的东西贴出来.英文水平一般,所以翻译的有点差,请海涵. Rac1 Activation Assay Kit被设计来鉴定贴壁细胞或非贴壁细胞的细胞裂解液中Rac活性数量(i.e. Rac-GTP, rather than the inactive Rac-GDP). 使用的glutathione (GST) pull-down方法，采用GST-agarose beads与有的包含74
Whole genome amplification protocol for ChIP-chip
-chip assay) allows a global analysis of binding sites for transcription factors and components of the transcriptional machinery, as well as of chromatin modification patterns. However, a single ChIP sample does not yield enough DNA for hybridization
, as expected for direct regulation of the promoter. Those TFZF s that did not transactivate the promoter in the reporter assay (such as 144-4, 144-5, and 144-13) showed different activation profiles that varied depending on the cell line examined
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